The published version of this manuscript can be found here: Sieler Jr M.J., Al-Samarrie C.E., Kasschau K.D., Kent M.L., Sharpton T.J.. Modeling the zebrafish gut microbiome’s resistance and sensitivity to climate change and infection. bioRxiv (2025). DOI: https://doi.org/10.1101/2025.03.28.644597
All code generated during this analysis is available in the GitHub Repository.
The raw sequence files generated during the current study are available at the NCBI Sequence Read Archive (SRA) project numbers: PRJNA1219243.
Fig.1 Experimental design showing treatments and husbandry events during the course of the study. Symbols indicate when an experimental event occurred at each time point. (1) 260 fish were assigned and acclimated to one of three water temperature groups (e.g., 28°C, 32°C, or 35°C) and reared from 0 to 164 days post fertilization (dpf). (2a) At 164 dpf (or 0 days post exposure; DPE), fecal collections were collected from a random selection of five fish per tank (n = 60). Additionally, histological and wet mount assessments were conducted on selected fish to assess presence of infection and infection burden. (1b) Afterwards, a cohort of fish from each water temperature group were exposed to Pseudocapillaria tomentosa. (3-6) Subsequent fecal samples were collected and histopathological assessments were conducted at 14 dpe (n = 54), (3) 21 dpe (n = 48), (4) 28 dpe (n = 47), and (5) 42 dpe (n = 51).
Fig. 2 Effects of water temperature on zebrafish gut microbiomes. (A) Simpson’s Index of diversity shows that gut microbiome diversity significantly differs between fish reared at 28°C and 35°C water temperatures. (B) Capscale ordination based on the Bray-Curtis dissimilarity of gut microbiome composition constrained on the main effect of temperature. The analysis shows that gut microbiome composition significantly differs between fish reared at different water temperatures. (C) Simpson’s Index of diversity shows microbial gut diversity increases with time from 0 days post exposure (dpe) to 42 dpe, irrespective of water temperature. (D) Capscale ordination of gut microbiome composition based on the Bray-Curtis dissimilarity constrained on the main effects of water temperature and time (days post exposure, dpe), and their interaction. The analysis shows that shows that gut microbiome composition differs between fish across time depending on water temperature. Ribbons and ellipses indicate 95% confidence interval. Only statistically significant relationships are shown. A “*” indicates statistical significance below the “0.05” level. Black arrows indicate direction of greatest change in the indicated by covariates.
Click on tabs to display tables. Scroll to see additional rows.
| GLM Results | |||||
| glm(Alpha.Score ~ Temperature); Unexposed fish | |||||
| term | estimate | std.error | statistic | p.value | p.adj.sig |
|---|---|---|---|---|---|
| Shannon | |||||
| (Intercept) | −0.754 | 0.091 | −8.309 | <0.001 | **** |
| Temperature32 | 0.051 | 0.127 | 0.400 | ≥0.25 | ns |
| Temperature35 | 0.054 | 0.129 | 0.421 | ≥0.25 | ns |
| Simpson | |||||
| (Intercept) | −0.101 | 0.092 | −1.103 | ≥0.25 | ns |
| Temperature32 | 0.132 | 0.128 | 1.030 | ≥0.25 | ns |
| Temperature35 | 0.340 | 0.131 | 2.591 | 0.010 | * |
| Richness | |||||
| (Intercept) | −0.231 | 0.075 | −3.079 | 0.002 | ** |
| Temperature32 | 0.047 | 0.105 | 0.446 | ≥0.25 | ns |
| Temperature35 | −0.473 | 0.110 | −4.299 | <0.001 | **** |
| Phylogenetic | |||||
| (Intercept) | −0.255 | 0.072 | −3.540 | <0.001 | *** |
| Temperature32 | 0.068 | 0.101 | 0.673 | ≥0.25 | ns |
| Temperature35 | −0.475 | 0.106 | −4.482 | <0.001 | **** |
| ANOVA of GLM | ||||
| ANOVA(GLM(Alpha.Score ~ Temperature), type = 2); Unexposed fish | ||||
| term | statistic | df | p.value | sig |
|---|---|---|---|---|
| Shannon | ||||
| Temperature | 0.225 | 2.000 | ≥0.25 | ns |
| Simpson | ||||
| Temperature | 6.830 | 2.000 | 0.033 | * |
| Richness | ||||
| Temperature | 27.512 | 2.000 | <0.001 | **** |
| Phylogenetic | ||||
| Temperature | 31.449 | 2.000 | <0.001 | **** |
| Pairwise Tukey's HSD, p.adj: Dunnett | ||||||||
| Tukey(Alpha.Score ~ Temperature); Unexposed fish | ||||||||
| term | .y. | group1 | group2 | estimate | std.error | statistic | adj.p.value | Variable |
|---|---|---|---|---|---|---|---|---|
| Shannon | ||||||||
| Temperature | Alpha.Score | 32 | 28 | 0.051 | 0.127 | 0.400 | ≥0.25 | Temperature |
| Temperature | Alpha.Score | 35 | 28 | 0.054 | 0.129 | 0.421 | ≥0.25 | Temperature |
| Temperature | Alpha.Score | 35 | 32 | 0.004 | 0.127 | 0.029 | ≥0.25 | Temperature |
| Simpson | ||||||||
| Temperature | Alpha.Score | 32 | 28 | 0.132 | 0.128 | 1.030 | ≥0.25 | Temperature |
| Temperature | Alpha.Score | 35 | 28 | 0.340 | 0.131 | 2.591 | 0.026 | Temperature |
| Temperature | Alpha.Score | 35 | 32 | 0.207 | 0.130 | 1.596 | 0.247 | Temperature |
| Richness | ||||||||
| Temperature | Alpha.Score | 32 | 28 | 0.047 | 0.105 | 0.446 | ≥0.25 | Temperature |
| Temperature | Alpha.Score | 35 | 28 | −0.473 | 0.110 | −4.299 | <0.001 | Temperature |
| Temperature | Alpha.Score | 35 | 32 | −0.520 | 0.109 | −4.768 | <0.001 | Temperature |
| Phylogenetic | ||||||||
| Temperature | Alpha.Score | 32 | 28 | 0.068 | 0.101 | 0.673 | ≥0.25 | Temperature |
| Temperature | Alpha.Score | 35 | 28 | −0.475 | 0.106 | −4.482 | <0.001 | Temperature |
| Temperature | Alpha.Score | 35 | 32 | −0.543 | 0.105 | −5.172 | <0.001 | Temperature |
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| ADONIS2 | ||||||
| adonis2(Beta Distance ~ Temperature); Unexposed fish | ||||||
| term | df | SumOfSqs | R2 | statistic | p.value | sig |
|---|---|---|---|---|---|---|
| bray | ||||||
| Temperature | 2.000 | 2.294 | 0.133 | 12.900 | 0.001 | *** |
| Residual | 168.000 | 14.936 | 0.867 | NA | NA | NA |
| Total | 170.000 | 17.229 | 1.000 | NA | NA | NA |
| canberra | ||||||
| Temperature | 2.000 | 3.862 | 0.099 | 9.178 | 0.001 | *** |
| Residual | 168.000 | 35.344 | 0.901 | NA | NA | NA |
| Total | 170.000 | 39.206 | 1.000 | NA | NA | NA |
| gunifrac | ||||||
| Temperature | 2.000 | 1.422 | 0.102 | 9.566 | 0.001 | *** |
| Residual | 168.000 | 12.485 | 0.898 | NA | NA | NA |
| Total | 170.000 | 13.907 | 1.000 | NA | NA | NA |
| ANOVA: Homogeneity of Dispersion | ||||||
| ANOVA(Beta Disperson ~ Temperature); Unexposed fish | ||||||
| term | df | sumsq | meansq | statistic | p.value | sig |
|---|---|---|---|---|---|---|
| bray | ||||||
| Temperature | 2.000 | 0.008 | 0.004 | 0.386 | ≥0.25 | ns |
| Residual | 168.000 | 1.771 | 0.011 | NA | NA | NA |
| canberra | ||||||
| Temperature | 2.000 | 0.008 | 0.004 | 0.873 | ≥0.25 | ns |
| Residual | 168.000 | 0.759 | 0.005 | NA | NA | NA |
| gunifrac | ||||||
| Temperature | 2.000 | 0.005 | 0.003 | 0.369 | ≥0.25 | ns |
| Residual | 168.000 | 1.143 | 0.007 | NA | NA | NA |
| Tukey: Homogeneity of Dispersion | ||||||||
| Tukey(Beta Disperson ~ Temperature); Unexposed fish | ||||||||
| .y. | term | group1 | group2 | estimate | conf.low | conf.high | adj.p.value | sig |
|---|---|---|---|---|---|---|---|---|
| bray | ||||||||
| Distance | Temperature | 32 | 28 | −0.011 | −0.056 | 0.034 | ≥0.25 | ns |
| Distance | Temperature | 35 | 28 | 0.005 | −0.041 | 0.051 | ≥0.25 | ns |
| Distance | Temperature | 35 | 32 | 0.016 | −0.029 | 0.062 | ≥0.25 | ns |
| canberra | ||||||||
| Distance | Temperature | 32 | 28 | −0.001 | −0.031 | 0.028 | ≥0.25 | ns |
| Distance | Temperature | 35 | 28 | 0.014 | −0.016 | 0.044 | ≥0.25 | ns |
| Distance | Temperature | 35 | 32 | 0.015 | −0.015 | 0.045 | ≥0.25 | ns |
| gunifrac | ||||||||
| Distance | Temperature | 32 | 28 | −0.013 | −0.049 | 0.023 | ≥0.25 | ns |
| Distance | Temperature | 35 | 28 | −0.004 | −0.040 | 0.033 | ≥0.25 | ns |
| Distance | Temperature | 35 | 32 | 0.009 | −0.027 | 0.046 | ≥0.25 | ns |
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| GLM Results | |||||
| glm(Alpha.Score ~ Temperature*DPE); Unexposed fish | |||||
| term | estimate | std.error | statistic | p.value | p.adj.sig |
|---|---|---|---|---|---|
| Shannon | |||||
| (Intercept) | −1.074 | 0.138 | −7.754 | <0.001 | **** |
| Temperature32 | 0.075 | 0.193 | 0.386 | ≥0.25 | ns |
| Temperature35 | 0.154 | 0.193 | 0.797 | ≥0.25 | ns |
| DPE | 0.018 | 0.006 | 3.086 | 0.002 | ** |
| Temperature32:DPE | −0.001 | 0.008 | −0.170 | ≥0.25 | ns |
| Temperature35:DPE | −0.005 | 0.008 | −0.585 | ≥0.25 | ns |
| Simpson | |||||
| (Intercept) | −0.433 | 0.134 | −3.245 | 0.001 | ** |
| Temperature32 | 0.157 | 0.186 | 0.841 | ≥0.25 | ns |
| Temperature35 | 0.408 | 0.187 | 2.177 | 0.031 | * |
| DPE | 0.019 | 0.006 | 3.293 | 0.001 | ** |
| Temperature32:DPE | −0.001 | 0.008 | −0.173 | ≥0.25 | ns |
| Temperature35:DPE | −0.003 | 0.008 | −0.343 | ≥0.25 | ns |
| Richness | |||||
| (Intercept) | −0.651 | 0.108 | −6.017 | <0.001 | **** |
| Temperature32 | 0.189 | 0.150 | 1.257 | 0.211 | ns |
| Temperature35 | −0.059 | 0.154 | −0.383 | ≥0.25 | ns |
| DPE | 0.024 | 0.005 | 5.137 | <0.001 | **** |
| Temperature32:DPE | −0.008 | 0.006 | −1.251 | 0.213 | ns |
| Temperature35:DPE | −0.024 | 0.007 | −3.476 | <0.001 | *** |
| Phylogenetic | |||||
| (Intercept) | −0.709 | 0.102 | −6.970 | <0.001 | **** |
| Temperature32 | 0.237 | 0.141 | 1.682 | 0.095 | ns |
| Temperature35 | 0.010 | 0.144 | 0.072 | ≥0.25 | ns |
| DPE | 0.026 | 0.004 | 5.907 | <0.001 | **** |
| Temperature32:DPE | −0.010 | 0.006 | −1.579 | 0.116 | ns |
| Temperature35:DPE | −0.028 | 0.006 | −4.344 | <0.001 | **** |
| ANOVA of GLM | ||||
| ANOVA(GLM(Alpha.Score ~ Temperature*Time), type = 2); Unexposed fish | ||||
| term | statistic | df | p.value | sig |
|---|---|---|---|---|
| Shannon | ||||
| Temperature | 0.322 | 2.000 | ≥0.25 | ns |
| DPE | 22.895 | 1.000 | <0.001 | **** |
| Temperature:DPE | 0.363 | 2.000 | ≥0.25 | ns |
| Simpson | ||||
| Temperature | 8.551 | 2.000 | 0.014 | * |
| DPE | 28.161 | 1.000 | <0.001 | **** |
| Temperature:DPE | 0.118 | 2.000 | ≥0.25 | ns |
| Richness | ||||
| Temperature | 31.759 | 2.000 | <0.001 | **** |
| DPE | 27.201 | 1.000 | <0.001 | **** |
| Temperature:DPE | 12.460 | 2.000 | 0.002 | ** |
| Phylogenetic | ||||
| Temperature | 38.358 | 2.000 | <0.001 | **** |
| DPE | 31.813 | 1.000 | <0.001 | **** |
| Temperature:DPE | 19.500 | 2.000 | <0.001 | **** |
| Pairwise Tukey's HSD, p.adj: Dunnett | ||||||||||
| Tukey(Alpha.Score ~ Temperature*Time); Unexposed fish | ||||||||||
| Temperature | .y. | term | group1 | group2 | estimate | std.error | statistic | adj.p.value | Variable | Group |
|---|---|---|---|---|---|---|---|---|---|---|
| Shannon | ||||||||||
| 28 | Alpha.Score | DPE | 14 | 0 | 1.065 | 0.294 | 3.625 | 0.003 | DPE | 28 |
| 28 | Alpha.Score | DPE | 21 | 0 | 0.722 | 0.281 | 2.566 | 0.076 | DPE | 28 |
| 28 | Alpha.Score | DPE | 28 | 0 | 1.187 | 0.274 | 4.335 | <0.001 | DPE | 28 |
| 28 | Alpha.Score | DPE | 42 | 0 | 0.548 | 0.296 | 1.853 | ≥0.25 | DPE | 28 |
| 28 | Alpha.Score | DPE | 21 | 14 | −0.343 | 0.317 | −1.083 | ≥0.25 | DPE | 28 |
| 28 | Alpha.Score | DPE | 28 | 14 | 0.122 | 0.310 | 0.392 | ≥0.25 | DPE | 28 |
| 28 | Alpha.Score | DPE | 42 | 14 | −0.517 | 0.330 | −1.567 | ≥0.25 | DPE | 28 |
| 28 | Alpha.Score | DPE | 28 | 21 | 0.465 | 0.299 | 1.558 | ≥0.25 | DPE | 28 |
| 28 | Alpha.Score | DPE | 42 | 21 | −0.174 | 0.319 | −0.545 | ≥0.25 | DPE | 28 |
| 28 | Alpha.Score | DPE | 42 | 28 | −0.639 | 0.312 | −2.045 | 0.243 | DPE | 28 |
| 32 | Alpha.Score | DPE | 14 | 0 | 0.006 | 0.250 | 0.022 | ≥0.25 | DPE | 32 |
| 32 | Alpha.Score | DPE | 21 | 0 | 0.112 | 0.247 | 0.452 | ≥0.25 | DPE | 32 |
| 32 | Alpha.Score | DPE | 28 | 0 | 0.420 | 0.248 | 1.695 | ≥0.25 | DPE | 32 |
| 32 | Alpha.Score | DPE | 42 | 0 | 0.694 | 0.236 | 2.943 | 0.026 | DPE | 32 |
| 32 | Alpha.Score | DPE | 21 | 14 | 0.106 | 0.286 | 0.371 | ≥0.25 | DPE | 32 |
| 32 | Alpha.Score | DPE | 28 | 14 | 0.414 | 0.287 | 1.445 | ≥0.25 | DPE | 32 |
| 32 | Alpha.Score | DPE | 42 | 14 | 0.688 | 0.276 | 2.490 | 0.092 | DPE | 32 |
| 32 | Alpha.Score | DPE | 28 | 21 | 0.308 | 0.283 | 1.087 | ≥0.25 | DPE | 32 |
| 32 | Alpha.Score | DPE | 42 | 21 | 0.582 | 0.273 | 2.131 | 0.205 | DPE | 32 |
| 32 | Alpha.Score | DPE | 42 | 28 | 0.274 | 0.274 | 1.001 | ≥0.25 | DPE | 32 |
| 35 | Alpha.Score | DPE | 14 | 0 | −0.006 | 0.210 | −0.027 | ≥0.25 | DPE | 35 |
| 35 | Alpha.Score | DPE | 21 | 0 | −0.249 | 0.218 | −1.145 | ≥0.25 | DPE | 35 |
| 35 | Alpha.Score | DPE | 28 | 0 | 0.232 | 0.204 | 1.138 | ≥0.25 | DPE | 35 |
| 35 | Alpha.Score | DPE | 42 | 0 | 0.661 | 0.207 | 3.194 | 0.012 | DPE | 35 |
| 35 | Alpha.Score | DPE | 21 | 14 | −0.243 | 0.253 | −0.962 | ≥0.25 | DPE | 35 |
| 35 | Alpha.Score | DPE | 28 | 14 | 0.238 | 0.242 | 0.985 | ≥0.25 | DPE | 35 |
| 35 | Alpha.Score | DPE | 42 | 14 | 0.667 | 0.244 | 2.733 | 0.049 | DPE | 35 |
| 35 | Alpha.Score | DPE | 28 | 21 | 0.481 | 0.248 | 1.938 | ≥0.25 | DPE | 35 |
| 35 | Alpha.Score | DPE | 42 | 21 | 0.910 | 0.251 | 3.628 | 0.003 | DPE | 35 |
| 35 | Alpha.Score | DPE | 42 | 28 | 0.429 | 0.239 | 1.792 | ≥0.25 | DPE | 35 |
| Simpson | ||||||||||
| 28 | Alpha.Score | DPE | 14 | 0 | 1.256 | 0.273 | 4.611 | <0.001 | DPE | 28 |
| 28 | Alpha.Score | DPE | 21 | 0 | 0.761 | 0.247 | 3.076 | 0.018 | DPE | 28 |
| 28 | Alpha.Score | DPE | 28 | 0 | 1.334 | 0.254 | 5.241 | <0.001 | DPE | 28 |
| 28 | Alpha.Score | DPE | 42 | 0 | 0.514 | 0.257 | 2.002 | ≥0.25 | DPE | 28 |
| 28 | Alpha.Score | DPE | 21 | 14 | −0.496 | 0.302 | −1.644 | ≥0.25 | DPE | 28 |
| 28 | Alpha.Score | DPE | 28 | 14 | 0.077 | 0.308 | 0.251 | ≥0.25 | DPE | 28 |
| 28 | Alpha.Score | DPE | 42 | 14 | −0.742 | 0.310 | −2.398 | 0.114 | DPE | 28 |
| 28 | Alpha.Score | DPE | 28 | 21 | 0.573 | 0.285 | 2.008 | ≥0.25 | DPE | 28 |
| 28 | Alpha.Score | DPE | 42 | 21 | −0.246 | 0.288 | −0.857 | ≥0.25 | DPE | 28 |
| 28 | Alpha.Score | DPE | 42 | 28 | −0.819 | 0.294 | −2.789 | 0.042 | DPE | 28 |
| 32 | Alpha.Score | DPE | 14 | 0 | 0.065 | 0.249 | 0.261 | ≥0.25 | DPE | 32 |
| 32 | Alpha.Score | DPE | 21 | 0 | 0.142 | 0.249 | 0.569 | ≥0.25 | DPE | 32 |
| 32 | Alpha.Score | DPE | 28 | 0 | 0.592 | 0.261 | 2.272 | 0.152 | DPE | 32 |
| 32 | Alpha.Score | DPE | 42 | 0 | 0.724 | 0.254 | 2.850 | 0.035 | DPE | 32 |
| 32 | Alpha.Score | DPE | 21 | 14 | 0.077 | 0.287 | 0.267 | ≥0.25 | DPE | 32 |
| 32 | Alpha.Score | DPE | 28 | 14 | 0.527 | 0.297 | 1.773 | ≥0.25 | DPE | 32 |
| 32 | Alpha.Score | DPE | 42 | 14 | 0.659 | 0.292 | 2.260 | 0.156 | DPE | 32 |
| 32 | Alpha.Score | DPE | 28 | 21 | 0.451 | 0.297 | 1.517 | ≥0.25 | DPE | 32 |
| 32 | Alpha.Score | DPE | 42 | 21 | 0.582 | 0.291 | 1.999 | ≥0.25 | DPE | 32 |
| 32 | Alpha.Score | DPE | 42 | 28 | 0.132 | 0.302 | 0.436 | ≥0.25 | DPE | 32 |
| 35 | Alpha.Score | DPE | 14 | 0 | 0.149 | 0.219 | 0.683 | ≥0.25 | DPE | 35 |
| 35 | Alpha.Score | DPE | 21 | 0 | −0.167 | 0.218 | −0.766 | ≥0.25 | DPE | 35 |
| 35 | Alpha.Score | DPE | 28 | 0 | 0.413 | 0.222 | 1.858 | ≥0.25 | DPE | 35 |
| 35 | Alpha.Score | DPE | 42 | 0 | 0.836 | 0.244 | 3.431 | 0.005 | DPE | 35 |
| 35 | Alpha.Score | DPE | 21 | 14 | −0.316 | 0.257 | −1.232 | ≥0.25 | DPE | 35 |
| 35 | Alpha.Score | DPE | 28 | 14 | 0.263 | 0.260 | 1.013 | ≥0.25 | DPE | 35 |
| 35 | Alpha.Score | DPE | 42 | 14 | 0.687 | 0.279 | 2.465 | 0.097 | DPE | 35 |
| 35 | Alpha.Score | DPE | 28 | 21 | 0.579 | 0.260 | 2.233 | 0.165 | DPE | 35 |
| 35 | Alpha.Score | DPE | 42 | 21 | 1.003 | 0.278 | 3.604 | 0.003 | DPE | 35 |
| 35 | Alpha.Score | DPE | 42 | 28 | 0.424 | 0.282 | 1.506 | ≥0.25 | DPE | 35 |
| Richness | ||||||||||
| 28 | Alpha.Score | DPE | 14 | 0 | 0.432 | 0.236 | 1.836 | ≥0.25 | DPE | 28 |
| 28 | Alpha.Score | DPE | 21 | 0 | 0.550 | 0.218 | 2.526 | 0.084 | DPE | 28 |
| 28 | Alpha.Score | DPE | 28 | 0 | 0.767 | 0.218 | 3.525 | 0.004 | DPE | 28 |
| 28 | Alpha.Score | DPE | 42 | 0 | 0.970 | 0.227 | 4.278 | <0.001 | DPE | 28 |
| 28 | Alpha.Score | DPE | 21 | 14 | 0.118 | 0.263 | 0.449 | ≥0.25 | DPE | 28 |
| 28 | Alpha.Score | DPE | 28 | 14 | 0.335 | 0.262 | 1.275 | ≥0.25 | DPE | 28 |
| 28 | Alpha.Score | DPE | 42 | 14 | 0.537 | 0.270 | 1.990 | ≥0.25 | DPE | 28 |
| 28 | Alpha.Score | DPE | 28 | 21 | 0.217 | 0.247 | 0.880 | ≥0.25 | DPE | 28 |
| 28 | Alpha.Score | DPE | 42 | 21 | 0.419 | 0.255 | 1.647 | ≥0.25 | DPE | 28 |
| 28 | Alpha.Score | DPE | 42 | 28 | 0.203 | 0.254 | 0.796 | ≥0.25 | DPE | 28 |
| 32 | Alpha.Score | DPE | 14 | 0 | −0.170 | 0.177 | −0.965 | ≥0.25 | DPE | 32 |
| 32 | Alpha.Score | DPE | 21 | 0 | −0.026 | 0.175 | −0.147 | ≥0.25 | DPE | 32 |
| 32 | Alpha.Score | DPE | 28 | 0 | 0.187 | 0.180 | 1.041 | ≥0.25 | DPE | 32 |
| 32 | Alpha.Score | DPE | 42 | 0 | 0.769 | 0.176 | 4.359 | <0.001 | DPE | 32 |
| 32 | Alpha.Score | DPE | 21 | 14 | 0.145 | 0.204 | 0.711 | ≥0.25 | DPE | 32 |
| 32 | Alpha.Score | DPE | 28 | 14 | 0.358 | 0.208 | 1.721 | ≥0.25 | DPE | 32 |
| 32 | Alpha.Score | DPE | 42 | 14 | 0.939 | 0.205 | 4.585 | <0.001 | DPE | 32 |
| 32 | Alpha.Score | DPE | 28 | 21 | 0.213 | 0.206 | 1.031 | ≥0.25 | DPE | 32 |
| 32 | Alpha.Score | DPE | 42 | 21 | 0.794 | 0.203 | 3.906 | <0.001 | DPE | 32 |
| 32 | Alpha.Score | DPE | 42 | 28 | 0.581 | 0.208 | 2.801 | 0.040 | DPE | 32 |
| 35 | Alpha.Score | DPE | 14 | 0 | −0.560 | 0.214 | −2.612 | 0.067 | DPE | 35 |
| 35 | Alpha.Score | DPE | 21 | 0 | −0.551 | 0.214 | −2.578 | 0.073 | DPE | 35 |
| 35 | Alpha.Score | DPE | 28 | 0 | 0.110 | 0.196 | 0.561 | ≥0.25 | DPE | 35 |
| 35 | Alpha.Score | DPE | 42 | 0 | 0.007 | 0.206 | 0.036 | ≥0.25 | DPE | 35 |
| 35 | Alpha.Score | DPE | 21 | 14 | 0.008 | 0.259 | 0.032 | ≥0.25 | DPE | 35 |
| 35 | Alpha.Score | DPE | 28 | 14 | 0.670 | 0.245 | 2.733 | 0.048 | DPE | 35 |
| 35 | Alpha.Score | DPE | 42 | 14 | 0.567 | 0.253 | 2.240 | 0.162 | DPE | 35 |
| 35 | Alpha.Score | DPE | 28 | 21 | 0.661 | 0.245 | 2.702 | 0.052 | DPE | 35 |
| 35 | Alpha.Score | DPE | 42 | 21 | 0.559 | 0.253 | 2.210 | 0.173 | DPE | 35 |
| 35 | Alpha.Score | DPE | 42 | 28 | −0.103 | 0.238 | −0.431 | ≥0.25 | DPE | 35 |
| Phylogenetic | ||||||||||
| 28 | Alpha.Score | DPE | 14 | 0 | 0.452 | 0.229 | 1.969 | ≥0.25 | DPE | 28 |
| 28 | Alpha.Score | DPE | 21 | 0 | 0.679 | 0.211 | 3.210 | 0.011 | DPE | 28 |
| 28 | Alpha.Score | DPE | 28 | 0 | 0.872 | 0.212 | 4.120 | <0.001 | DPE | 28 |
| 28 | Alpha.Score | DPE | 42 | 0 | 1.012 | 0.220 | 4.602 | <0.001 | DPE | 28 |
| 28 | Alpha.Score | DPE | 21 | 14 | 0.227 | 0.254 | 0.893 | ≥0.25 | DPE | 28 |
| 28 | Alpha.Score | DPE | 28 | 14 | 0.420 | 0.254 | 1.651 | ≥0.25 | DPE | 28 |
| 28 | Alpha.Score | DPE | 42 | 14 | 0.560 | 0.261 | 2.142 | 0.200 | DPE | 28 |
| 28 | Alpha.Score | DPE | 28 | 21 | 0.193 | 0.238 | 0.809 | ≥0.25 | DPE | 28 |
| 28 | Alpha.Score | DPE | 42 | 21 | 0.333 | 0.246 | 1.353 | ≥0.25 | DPE | 28 |
| 28 | Alpha.Score | DPE | 42 | 28 | 0.140 | 0.246 | 0.569 | ≥0.25 | DPE | 28 |
| 32 | Alpha.Score | DPE | 14 | 0 | −0.030 | 0.164 | −0.181 | ≥0.25 | DPE | 32 |
| 32 | Alpha.Score | DPE | 21 | 0 | 0.154 | 0.163 | 0.945 | ≥0.25 | DPE | 32 |
| 32 | Alpha.Score | DPE | 28 | 0 | 0.260 | 0.168 | 1.550 | ≥0.25 | DPE | 32 |
| 32 | Alpha.Score | DPE | 42 | 0 | 0.753 | 0.164 | 4.594 | <0.001 | DPE | 32 |
| 32 | Alpha.Score | DPE | 21 | 14 | 0.183 | 0.188 | 0.974 | ≥0.25 | DPE | 32 |
| 32 | Alpha.Score | DPE | 28 | 14 | 0.290 | 0.193 | 1.503 | ≥0.25 | DPE | 32 |
| 32 | Alpha.Score | DPE | 42 | 14 | 0.783 | 0.190 | 4.130 | <0.001 | DPE | 32 |
| 32 | Alpha.Score | DPE | 28 | 21 | 0.107 | 0.192 | 0.556 | ≥0.25 | DPE | 32 |
| 32 | Alpha.Score | DPE | 42 | 21 | 0.599 | 0.188 | 3.185 | 0.013 | DPE | 32 |
| 32 | Alpha.Score | DPE | 42 | 28 | 0.493 | 0.193 | 2.555 | 0.078 | DPE | 32 |
| 35 | Alpha.Score | DPE | 14 | 0 | −0.393 | 0.204 | −1.929 | ≥0.25 | DPE | 35 |
| 35 | Alpha.Score | DPE | 21 | 0 | −0.453 | 0.206 | −2.198 | 0.177 | DPE | 35 |
| 35 | Alpha.Score | DPE | 28 | 0 | 0.090 | 0.192 | 0.468 | ≥0.25 | DPE | 35 |
| 35 | Alpha.Score | DPE | 42 | 0 | −0.111 | 0.204 | −0.542 | ≥0.25 | DPE | 35 |
| 35 | Alpha.Score | DPE | 21 | 14 | −0.059 | 0.246 | −0.241 | ≥0.25 | DPE | 35 |
| 35 | Alpha.Score | DPE | 28 | 14 | 0.483 | 0.235 | 2.057 | 0.235 | DPE | 35 |
| 35 | Alpha.Score | DPE | 42 | 14 | 0.282 | 0.245 | 1.153 | ≥0.25 | DPE | 35 |
| 35 | Alpha.Score | DPE | 28 | 21 | 0.542 | 0.237 | 2.292 | 0.145 | DPE | 35 |
| 35 | Alpha.Score | DPE | 42 | 21 | 0.342 | 0.247 | 1.386 | ≥0.25 | DPE | 35 |
| 35 | Alpha.Score | DPE | 42 | 28 | −0.200 | 0.235 | −0.853 | ≥0.25 | DPE | 35 |
Click on tabs to display tables. Scroll to see additional rows.
| ADONIS2 | ||||||
| adonis2(Beta Distance ~ Temperature*DPE); Unexposed fish | ||||||
| term | df | SumOfSqs | R2 | statistic | p.value | sig |
|---|---|---|---|---|---|---|
| bray | ||||||
| Temperature | 2.000 | 2.294 | 0.133 | 16.443 | 0.001 | *** |
| DPE | 1.000 | 2.531 | 0.147 | 36.284 | 0.001 | *** |
| Temperature:DPE | 2.000 | 0.896 | 0.052 | 6.425 | 0.001 | *** |
| Residual | 165.000 | 11.509 | 0.668 | NA | NA | NA |
| Total | 170.000 | 17.229 | 1.000 | NA | NA | NA |
| canberra | ||||||
| Temperature | 2.000 | 3.862 | 0.099 | 10.667 | 0.001 | *** |
| DPE | 1.000 | 3.655 | 0.093 | 20.192 | 0.001 | *** |
| Temperature:DPE | 2.000 | 1.820 | 0.046 | 5.026 | 0.001 | *** |
| Residual | 165.000 | 29.869 | 0.762 | NA | NA | NA |
| Total | 170.000 | 39.206 | 1.000 | NA | NA | NA |
| gunifrac | ||||||
| Temperature | 2.000 | 1.422 | 0.102 | 11.984 | 0.001 | *** |
| DPE | 1.000 | 2.123 | 0.153 | 35.785 | 0.001 | *** |
| Temperature:DPE | 2.000 | 0.574 | 0.041 | 4.840 | 0.001 | *** |
| Residual | 165.000 | 9.788 | 0.704 | NA | NA | NA |
| Total | 170.000 | 13.907 | 1.000 | NA | NA | NA |
| ANOVA: Homogeneity of Dispersion | ||||||
| ANOVA(Beta Disperson ~ Temperature); Unexposed fish | ||||||
| term | df | sumsq | meansq | statistic | p.value | sig |
|---|---|---|---|---|---|---|
| bray | ||||||
| Temp.DPE | 14.000 | 0.290 | 0.021 | 2.348 | 0.006 | ** |
| Residual | 156.000 | 1.376 | 0.009 | NA | NA | NA |
| canberra | ||||||
| Temp.DPE | 14.000 | 0.250 | 0.018 | 3.344 | <0.001 | *** |
| Residual | 156.000 | 0.831 | 0.005 | NA | NA | NA |
| gunifrac | ||||||
| Temp.DPE | 14.000 | 0.249 | 0.018 | 2.986 | <0.001 | *** |
| Residual | 156.000 | 0.929 | 0.006 | NA | NA | NA |
| Tukey: Homogeneity of Dispersion | ||||||||
| Tukey(Beta Disperson ~ Temperature*DPE); Unexposed fish | ||||||||
| .y. | term | group1 | group2 | estimate | conf.low | conf.high | adj.p.value | sig |
|---|---|---|---|---|---|---|---|---|
| bray | ||||||||
| Distance | Temp.DPE | 28°C__14DPE | 28°C__0DPE | 0.024 | −0.112 | 0.159 | ≥0.25 | ns |
| Distance | Temp.DPE | 28°C__21DPE | 28°C__0DPE | 0.052 | −0.074 | 0.177 | ≥0.25 | ns |
| Distance | Temp.DPE | 28°C__28DPE | 28°C__0DPE | 0.007 | −0.118 | 0.133 | ≥0.25 | ns |
| Distance | Temp.DPE | 28°C__42DPE | 28°C__0DPE | −0.017 | −0.147 | 0.113 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__0DPE | 28°C__0DPE | 0.097 | −0.005 | 0.199 | 0.085 | ns |
| Distance | Temp.DPE | 32°C__14DPE | 28°C__0DPE | 0.041 | −0.085 | 0.166 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__21DPE | 28°C__0DPE | −0.018 | −0.143 | 0.108 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__28DPE | 28°C__0DPE | 0.028 | −0.102 | 0.158 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__42DPE | 28°C__0DPE | 0.017 | −0.108 | 0.142 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__0DPE | 28°C__0DPE | 0.044 | −0.058 | 0.146 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__14DPE | 28°C__0DPE | 0.004 | −0.126 | 0.134 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__21DPE | 28°C__0DPE | −0.004 | −0.134 | 0.126 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__28DPE | 28°C__0DPE | 0.033 | −0.097 | 0.162 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__42DPE | 28°C__0DPE | 0.146 | 0.011 | 0.282 | 0.021 | * |
| Distance | Temp.DPE | 28°C__21DPE | 28°C__14DPE | 0.028 | −0.126 | 0.181 | ≥0.25 | ns |
| Distance | Temp.DPE | 28°C__28DPE | 28°C__14DPE | −0.016 | −0.170 | 0.137 | ≥0.25 | ns |
| Distance | Temp.DPE | 28°C__42DPE | 28°C__14DPE | −0.040 | −0.198 | 0.117 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__0DPE | 28°C__14DPE | 0.073 | −0.062 | 0.209 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__14DPE | 28°C__14DPE | 0.017 | −0.136 | 0.171 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__21DPE | 28°C__14DPE | −0.041 | −0.195 | 0.112 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__28DPE | 28°C__14DPE | 0.005 | −0.153 | 0.162 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__42DPE | 28°C__14DPE | −0.006 | −0.160 | 0.147 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__0DPE | 28°C__14DPE | 0.020 | −0.115 | 0.156 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__14DPE | 28°C__14DPE | −0.019 | −0.176 | 0.138 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__21DPE | 28°C__14DPE | −0.028 | −0.185 | 0.129 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__28DPE | 28°C__14DPE | 0.009 | −0.148 | 0.166 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__42DPE | 28°C__14DPE | 0.123 | −0.039 | 0.284 | ≥0.25 | ns |
| Distance | Temp.DPE | 28°C__28DPE | 28°C__21DPE | −0.044 | −0.189 | 0.101 | ≥0.25 | ns |
| Distance | Temp.DPE | 28°C__42DPE | 28°C__21DPE | −0.068 | −0.217 | 0.081 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__0DPE | 28°C__21DPE | 0.045 | −0.080 | 0.171 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__14DPE | 28°C__21DPE | −0.011 | −0.156 | 0.134 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__21DPE | 28°C__21DPE | −0.069 | −0.214 | 0.076 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__28DPE | 28°C__21DPE | −0.023 | −0.172 | 0.125 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__42DPE | 28°C__21DPE | −0.034 | −0.179 | 0.110 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__0DPE | 28°C__21DPE | −0.008 | −0.133 | 0.118 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__14DPE | 28°C__21DPE | −0.047 | −0.196 | 0.102 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__21DPE | 28°C__21DPE | −0.056 | −0.205 | 0.093 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__28DPE | 28°C__21DPE | −0.019 | −0.168 | 0.130 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__42DPE | 28°C__21DPE | 0.095 | −0.059 | 0.248 | ≥0.25 | ns |
| Distance | Temp.DPE | 28°C__42DPE | 28°C__28DPE | −0.024 | −0.173 | 0.125 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__0DPE | 28°C__28DPE | 0.089 | −0.036 | 0.215 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__14DPE | 28°C__28DPE | 0.033 | −0.111 | 0.178 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__21DPE | 28°C__28DPE | −0.025 | −0.170 | 0.120 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__28DPE | 28°C__28DPE | 0.021 | −0.128 | 0.169 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__42DPE | 28°C__28DPE | 0.010 | −0.135 | 0.154 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__0DPE | 28°C__28DPE | 0.036 | −0.089 | 0.162 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__14DPE | 28°C__28DPE | −0.003 | −0.152 | 0.146 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__21DPE | 28°C__28DPE | −0.012 | −0.161 | 0.137 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__28DPE | 28°C__28DPE | 0.025 | −0.124 | 0.174 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__42DPE | 28°C__28DPE | 0.139 | −0.015 | 0.292 | 0.125 | ns |
| Distance | Temp.DPE | 32°C__0DPE | 28°C__42DPE | 0.114 | −0.016 | 0.243 | 0.161 | ns |
| Distance | Temp.DPE | 32°C__14DPE | 28°C__42DPE | 0.057 | −0.091 | 0.206 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__21DPE | 28°C__42DPE | −0.001 | −0.150 | 0.148 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__28DPE | 28°C__42DPE | 0.045 | −0.108 | 0.197 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__42DPE | 28°C__42DPE | 0.034 | −0.115 | 0.183 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__0DPE | 28°C__42DPE | 0.061 | −0.069 | 0.191 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__14DPE | 28°C__42DPE | 0.021 | −0.131 | 0.174 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__21DPE | 28°C__42DPE | 0.012 | −0.140 | 0.165 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__28DPE | 28°C__42DPE | 0.049 | −0.103 | 0.202 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__42DPE | 28°C__42DPE | 0.163 | 0.006 | 0.320 | 0.034 | * |
| Distance | Temp.DPE | 32°C__14DPE | 32°C__0DPE | −0.056 | −0.182 | 0.069 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__21DPE | 32°C__0DPE | −0.114 | −0.240 | 0.011 | 0.115 | ns |
| Distance | Temp.DPE | 32°C__28DPE | 32°C__0DPE | −0.069 | −0.199 | 0.061 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__42DPE | 32°C__0DPE | −0.080 | −0.205 | 0.046 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__0DPE | 32°C__0DPE | −0.053 | −0.155 | 0.049 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__14DPE | 32°C__0DPE | −0.092 | −0.222 | 0.037 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__21DPE | 32°C__0DPE | −0.101 | −0.231 | 0.029 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__28DPE | 32°C__0DPE | −0.064 | −0.194 | 0.066 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__42DPE | 32°C__0DPE | 0.049 | −0.086 | 0.185 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__21DPE | 32°C__14DPE | −0.058 | −0.203 | 0.086 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__28DPE | 32°C__14DPE | −0.013 | −0.161 | 0.136 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__42DPE | 32°C__14DPE | −0.024 | −0.168 | 0.121 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__0DPE | 32°C__14DPE | 0.003 | −0.122 | 0.129 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__14DPE | 32°C__14DPE | −0.036 | −0.185 | 0.112 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__21DPE | 32°C__14DPE | −0.045 | −0.194 | 0.104 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__28DPE | 32°C__14DPE | −0.008 | −0.157 | 0.141 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__42DPE | 32°C__14DPE | 0.105 | −0.048 | 0.259 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__28DPE | 32°C__21DPE | 0.046 | −0.103 | 0.194 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__42DPE | 32°C__21DPE | 0.035 | −0.110 | 0.179 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__0DPE | 32°C__21DPE | 0.061 | −0.064 | 0.187 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__14DPE | 32°C__21DPE | 0.022 | −0.127 | 0.171 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__21DPE | 32°C__21DPE | 0.013 | −0.136 | 0.162 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__28DPE | 32°C__21DPE | 0.050 | −0.099 | 0.199 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__42DPE | 32°C__21DPE | 0.164 | 0.010 | 0.317 | 0.025 | * |
| Distance | Temp.DPE | 32°C__42DPE | 32°C__28DPE | −0.011 | −0.160 | 0.138 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__0DPE | 32°C__28DPE | 0.016 | −0.114 | 0.146 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__14DPE | 32°C__28DPE | −0.024 | −0.176 | 0.129 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__21DPE | 32°C__28DPE | −0.033 | −0.185 | 0.120 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__28DPE | 32°C__28DPE | 0.004 | −0.148 | 0.157 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__42DPE | 32°C__28DPE | 0.118 | −0.039 | 0.275 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__0DPE | 32°C__42DPE | 0.027 | −0.099 | 0.152 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__14DPE | 32°C__42DPE | −0.013 | −0.161 | 0.136 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__21DPE | 32°C__42DPE | −0.021 | −0.170 | 0.127 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__28DPE | 32°C__42DPE | 0.015 | −0.133 | 0.164 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__42DPE | 32°C__42DPE | 0.129 | −0.024 | 0.283 | 0.209 | ns |
| Distance | Temp.DPE | 35°C__14DPE | 35°C__0DPE | −0.039 | −0.169 | 0.090 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__21DPE | 35°C__0DPE | −0.048 | −0.178 | 0.082 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__28DPE | 35°C__0DPE | −0.011 | −0.141 | 0.119 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__42DPE | 35°C__0DPE | 0.102 | −0.033 | 0.238 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__21DPE | 35°C__14DPE | −0.009 | −0.161 | 0.144 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__28DPE | 35°C__14DPE | 0.028 | −0.124 | 0.181 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__42DPE | 35°C__14DPE | 0.142 | −0.016 | 0.299 | 0.127 | ns |
| Distance | Temp.DPE | 35°C__28DPE | 35°C__21DPE | 0.037 | −0.116 | 0.190 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__42DPE | 35°C__21DPE | 0.151 | −0.007 | 0.308 | 0.076 | ns |
| Distance | Temp.DPE | 35°C__42DPE | 35°C__28DPE | 0.114 | −0.044 | 0.271 | ≥0.25 | ns |
| canberra | ||||||||
| Distance | Temp.DPE | 28°C__14DPE | 28°C__0DPE | −0.033 | −0.138 | 0.072 | ≥0.25 | ns |
| Distance | Temp.DPE | 28°C__21DPE | 28°C__0DPE | −0.005 | −0.103 | 0.092 | ≥0.25 | ns |
| Distance | Temp.DPE | 28°C__28DPE | 28°C__0DPE | −0.074 | −0.171 | 0.024 | ≥0.25 | ns |
| Distance | Temp.DPE | 28°C__42DPE | 28°C__0DPE | −0.069 | −0.170 | 0.032 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__0DPE | 28°C__0DPE | 0.035 | −0.045 | 0.115 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__14DPE | 28°C__0DPE | −0.021 | −0.119 | 0.076 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__21DPE | 28°C__0DPE | −0.070 | −0.167 | 0.028 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__28DPE | 28°C__0DPE | −0.056 | −0.157 | 0.045 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__42DPE | 28°C__0DPE | −0.068 | −0.165 | 0.030 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__0DPE | 28°C__0DPE | 0.008 | −0.071 | 0.088 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__14DPE | 28°C__0DPE | −0.041 | −0.142 | 0.060 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__21DPE | 28°C__0DPE | −0.047 | −0.148 | 0.054 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__28DPE | 28°C__0DPE | −0.052 | −0.153 | 0.049 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__42DPE | 28°C__0DPE | 0.045 | −0.061 | 0.150 | ≥0.25 | ns |
| Distance | Temp.DPE | 28°C__21DPE | 28°C__14DPE | 0.028 | −0.091 | 0.147 | ≥0.25 | ns |
| Distance | Temp.DPE | 28°C__28DPE | 28°C__14DPE | −0.040 | −0.160 | 0.079 | ≥0.25 | ns |
| Distance | Temp.DPE | 28°C__42DPE | 28°C__14DPE | −0.035 | −0.158 | 0.087 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__0DPE | 28°C__14DPE | 0.068 | −0.037 | 0.174 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__14DPE | 28°C__14DPE | 0.012 | −0.107 | 0.131 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__21DPE | 28°C__14DPE | −0.037 | −0.156 | 0.083 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__28DPE | 28°C__14DPE | −0.023 | −0.145 | 0.099 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__42DPE | 28°C__14DPE | −0.034 | −0.154 | 0.085 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__0DPE | 28°C__14DPE | 0.042 | −0.064 | 0.147 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__14DPE | 28°C__14DPE | −0.008 | −0.130 | 0.115 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__21DPE | 28°C__14DPE | −0.013 | −0.136 | 0.109 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__28DPE | 28°C__14DPE | −0.019 | −0.141 | 0.103 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__42DPE | 28°C__14DPE | 0.078 | −0.048 | 0.204 | ≥0.25 | ns |
| Distance | Temp.DPE | 28°C__28DPE | 28°C__21DPE | −0.068 | −0.181 | 0.044 | ≥0.25 | ns |
| Distance | Temp.DPE | 28°C__42DPE | 28°C__21DPE | −0.063 | −0.179 | 0.052 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__0DPE | 28°C__21DPE | 0.040 | −0.057 | 0.138 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__14DPE | 28°C__21DPE | −0.016 | −0.128 | 0.097 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__21DPE | 28°C__21DPE | −0.065 | −0.177 | 0.048 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__28DPE | 28°C__21DPE | −0.051 | −0.167 | 0.065 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__42DPE | 28°C__21DPE | −0.062 | −0.175 | 0.050 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__0DPE | 28°C__21DPE | 0.014 | −0.084 | 0.111 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__14DPE | 28°C__21DPE | −0.036 | −0.151 | 0.080 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__21DPE | 28°C__21DPE | −0.041 | −0.157 | 0.074 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__28DPE | 28°C__21DPE | −0.047 | −0.162 | 0.069 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__42DPE | 28°C__21DPE | 0.050 | −0.070 | 0.169 | ≥0.25 | ns |
| Distance | Temp.DPE | 28°C__42DPE | 28°C__28DPE | 0.005 | −0.110 | 0.121 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__0DPE | 28°C__28DPE | 0.109 | 0.011 | 0.206 | 0.014 | * |
| Distance | Temp.DPE | 32°C__14DPE | 28°C__28DPE | 0.052 | −0.060 | 0.165 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__21DPE | 28°C__28DPE | 0.004 | −0.109 | 0.116 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__28DPE | 28°C__28DPE | 0.017 | −0.098 | 0.133 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__42DPE | 28°C__28DPE | 0.006 | −0.106 | 0.119 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__0DPE | 28°C__28DPE | 0.082 | −0.015 | 0.180 | 0.206 | ns |
| Distance | Temp.DPE | 35°C__14DPE | 28°C__28DPE | 0.033 | −0.083 | 0.148 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__21DPE | 28°C__28DPE | 0.027 | −0.088 | 0.143 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__28DPE | 28°C__28DPE | 0.021 | −0.094 | 0.137 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__42DPE | 28°C__28DPE | 0.118 | −0.001 | 0.237 | 0.055 | ns |
| Distance | Temp.DPE | 32°C__0DPE | 28°C__42DPE | 0.104 | 0.003 | 0.205 | 0.038 | * |
| Distance | Temp.DPE | 32°C__14DPE | 28°C__42DPE | 0.047 | −0.068 | 0.163 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__21DPE | 28°C__42DPE | −0.001 | −0.117 | 0.114 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__28DPE | 28°C__42DPE | 0.012 | −0.106 | 0.131 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__42DPE | 28°C__42DPE | 0.001 | −0.115 | 0.117 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__0DPE | 28°C__42DPE | 0.077 | −0.024 | 0.178 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__14DPE | 28°C__42DPE | 0.028 | −0.091 | 0.146 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__21DPE | 28°C__42DPE | 0.022 | −0.097 | 0.141 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__28DPE | 28°C__42DPE | 0.016 | −0.102 | 0.135 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__42DPE | 28°C__42DPE | 0.113 | −0.009 | 0.235 | 0.104 | ns |
| Distance | Temp.DPE | 32°C__14DPE | 32°C__0DPE | −0.056 | −0.154 | 0.041 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__21DPE | 32°C__0DPE | −0.105 | −0.202 | −0.007 | 0.022 | * |
| Distance | Temp.DPE | 32°C__28DPE | 32°C__0DPE | −0.091 | −0.192 | 0.010 | 0.125 | ns |
| Distance | Temp.DPE | 32°C__42DPE | 32°C__0DPE | −0.103 | −0.200 | −0.005 | 0.029 | * |
| Distance | Temp.DPE | 35°C__0DPE | 32°C__0DPE | −0.027 | −0.106 | 0.053 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__14DPE | 32°C__0DPE | −0.076 | −0.177 | 0.025 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__21DPE | 32°C__0DPE | −0.082 | −0.183 | 0.019 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__28DPE | 32°C__0DPE | −0.087 | −0.188 | 0.014 | 0.175 | ns |
| Distance | Temp.DPE | 35°C__42DPE | 32°C__0DPE | 0.009 | −0.096 | 0.115 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__21DPE | 32°C__14DPE | −0.049 | −0.161 | 0.064 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__28DPE | 32°C__14DPE | −0.035 | −0.151 | 0.081 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__42DPE | 32°C__14DPE | −0.046 | −0.159 | 0.066 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__0DPE | 32°C__14DPE | 0.030 | −0.068 | 0.127 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__14DPE | 32°C__14DPE | −0.020 | −0.135 | 0.096 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__21DPE | 32°C__14DPE | −0.025 | −0.141 | 0.090 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__28DPE | 32°C__14DPE | −0.031 | −0.147 | 0.085 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__42DPE | 32°C__14DPE | 0.066 | −0.054 | 0.185 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__28DPE | 32°C__21DPE | 0.014 | −0.102 | 0.129 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__42DPE | 32°C__21DPE | 0.002 | −0.110 | 0.115 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__0DPE | 32°C__21DPE | 0.078 | −0.019 | 0.176 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__14DPE | 32°C__21DPE | 0.029 | −0.087 | 0.145 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__21DPE | 32°C__21DPE | 0.023 | −0.092 | 0.139 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__28DPE | 32°C__21DPE | 0.018 | −0.098 | 0.133 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__42DPE | 32°C__21DPE | 0.114 | −0.005 | 0.234 | 0.076 | ns |
| Distance | Temp.DPE | 32°C__42DPE | 32°C__28DPE | −0.011 | −0.127 | 0.104 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__0DPE | 32°C__28DPE | 0.065 | −0.036 | 0.166 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__14DPE | 32°C__28DPE | 0.015 | −0.103 | 0.134 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__21DPE | 32°C__28DPE | 0.010 | −0.109 | 0.128 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__28DPE | 32°C__28DPE | 0.004 | −0.115 | 0.123 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__42DPE | 32°C__28DPE | 0.101 | −0.022 | 0.223 | 0.237 | ns |
| Distance | Temp.DPE | 35°C__0DPE | 32°C__42DPE | 0.076 | −0.021 | 0.173 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__14DPE | 32°C__42DPE | 0.027 | −0.089 | 0.142 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__21DPE | 32°C__42DPE | 0.021 | −0.095 | 0.137 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__28DPE | 32°C__42DPE | 0.015 | −0.100 | 0.131 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__42DPE | 32°C__42DPE | 0.112 | −0.007 | 0.231 | 0.091 | ns |
| Distance | Temp.DPE | 35°C__14DPE | 35°C__0DPE | −0.049 | −0.150 | 0.052 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__21DPE | 35°C__0DPE | −0.055 | −0.156 | 0.046 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__28DPE | 35°C__0DPE | −0.061 | −0.162 | 0.040 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__42DPE | 35°C__0DPE | 0.036 | −0.069 | 0.141 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__21DPE | 35°C__14DPE | −0.006 | −0.124 | 0.113 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__28DPE | 35°C__14DPE | −0.011 | −0.130 | 0.107 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__42DPE | 35°C__14DPE | 0.085 | −0.037 | 0.208 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__28DPE | 35°C__21DPE | −0.006 | −0.124 | 0.113 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__42DPE | 35°C__21DPE | 0.091 | −0.031 | 0.213 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__42DPE | 35°C__28DPE | 0.097 | −0.026 | 0.219 | ≥0.25 | ns |
| gunifrac | ||||||||
| Distance | Temp.DPE | 28°C__14DPE | 28°C__0DPE | −0.005 | −0.116 | 0.107 | ≥0.25 | ns |
| Distance | Temp.DPE | 28°C__21DPE | 28°C__0DPE | 0.038 | −0.065 | 0.141 | ≥0.25 | ns |
| Distance | Temp.DPE | 28°C__28DPE | 28°C__0DPE | −0.010 | −0.113 | 0.093 | ≥0.25 | ns |
| Distance | Temp.DPE | 28°C__42DPE | 28°C__0DPE | −0.033 | −0.140 | 0.073 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__0DPE | 28°C__0DPE | 0.067 | −0.017 | 0.151 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__14DPE | 28°C__0DPE | 0.018 | −0.085 | 0.121 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__21DPE | 28°C__0DPE | −0.023 | −0.126 | 0.080 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__28DPE | 28°C__0DPE | −0.014 | −0.121 | 0.093 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__42DPE | 28°C__0DPE | −0.026 | −0.129 | 0.077 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__0DPE | 28°C__0DPE | 0.029 | −0.055 | 0.113 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__14DPE | 28°C__0DPE | −0.015 | −0.122 | 0.092 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__21DPE | 28°C__0DPE | −0.020 | −0.127 | 0.087 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__28DPE | 28°C__0DPE | −0.065 | −0.171 | 0.042 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__42DPE | 28°C__0DPE | 0.091 | −0.020 | 0.202 | 0.245 | ns |
| Distance | Temp.DPE | 28°C__21DPE | 28°C__14DPE | 0.042 | −0.084 | 0.168 | ≥0.25 | ns |
| Distance | Temp.DPE | 28°C__28DPE | 28°C__14DPE | −0.005 | −0.131 | 0.121 | ≥0.25 | ns |
| Distance | Temp.DPE | 28°C__42DPE | 28°C__14DPE | −0.029 | −0.158 | 0.100 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__0DPE | 28°C__14DPE | 0.072 | −0.040 | 0.183 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__14DPE | 28°C__14DPE | 0.022 | −0.104 | 0.148 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__21DPE | 28°C__14DPE | −0.019 | −0.145 | 0.107 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__28DPE | 28°C__14DPE | −0.009 | −0.138 | 0.120 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__42DPE | 28°C__14DPE | −0.021 | −0.147 | 0.105 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__0DPE | 28°C__14DPE | 0.033 | −0.078 | 0.145 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__14DPE | 28°C__14DPE | −0.010 | −0.139 | 0.119 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__21DPE | 28°C__14DPE | −0.015 | −0.145 | 0.114 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__28DPE | 28°C__14DPE | −0.060 | −0.189 | 0.069 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__42DPE | 28°C__14DPE | 0.096 | −0.037 | 0.229 | ≥0.25 | ns |
| Distance | Temp.DPE | 28°C__28DPE | 28°C__21DPE | −0.047 | −0.166 | 0.072 | ≥0.25 | ns |
| Distance | Temp.DPE | 28°C__42DPE | 28°C__21DPE | −0.071 | −0.193 | 0.051 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__0DPE | 28°C__21DPE | 0.029 | −0.074 | 0.132 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__14DPE | 28°C__21DPE | −0.020 | −0.139 | 0.099 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__21DPE | 28°C__21DPE | −0.061 | −0.180 | 0.058 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__28DPE | 28°C__21DPE | −0.051 | −0.174 | 0.071 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__42DPE | 28°C__21DPE | −0.063 | −0.182 | 0.056 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__0DPE | 28°C__21DPE | −0.009 | −0.112 | 0.094 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__14DPE | 28°C__21DPE | −0.052 | −0.175 | 0.070 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__21DPE | 28°C__21DPE | −0.058 | −0.180 | 0.065 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__28DPE | 28°C__21DPE | −0.102 | −0.224 | 0.020 | 0.216 | ns |
| Distance | Temp.DPE | 35°C__42DPE | 28°C__21DPE | 0.054 | −0.073 | 0.180 | ≥0.25 | ns |
| Distance | Temp.DPE | 28°C__42DPE | 28°C__28DPE | −0.024 | −0.146 | 0.098 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__0DPE | 28°C__28DPE | 0.077 | −0.026 | 0.180 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__14DPE | 28°C__28DPE | 0.027 | −0.092 | 0.146 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__21DPE | 28°C__28DPE | −0.014 | −0.133 | 0.105 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__28DPE | 28°C__28DPE | −0.004 | −0.126 | 0.118 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__42DPE | 28°C__28DPE | −0.016 | −0.135 | 0.103 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__0DPE | 28°C__28DPE | 0.038 | −0.065 | 0.142 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__14DPE | 28°C__28DPE | −0.005 | −0.127 | 0.117 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__21DPE | 28°C__28DPE | −0.010 | −0.132 | 0.112 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__28DPE | 28°C__28DPE | −0.055 | −0.177 | 0.067 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__42DPE | 28°C__28DPE | 0.101 | −0.025 | 0.227 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__0DPE | 28°C__42DPE | 0.100 | −0.006 | 0.207 | 0.089 | ns |
| Distance | Temp.DPE | 32°C__14DPE | 28°C__42DPE | 0.051 | −0.071 | 0.173 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__21DPE | 28°C__42DPE | 0.010 | −0.112 | 0.132 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__28DPE | 28°C__42DPE | 0.020 | −0.106 | 0.145 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__42DPE | 28°C__42DPE | 0.008 | −0.114 | 0.130 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__0DPE | 28°C__42DPE | 0.062 | −0.045 | 0.169 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__14DPE | 28°C__42DPE | 0.019 | −0.107 | 0.144 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__21DPE | 28°C__42DPE | 0.014 | −0.112 | 0.139 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__28DPE | 28°C__42DPE | −0.031 | −0.157 | 0.094 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__42DPE | 28°C__42DPE | 0.125 | −0.005 | 0.254 | 0.071 | ns |
| Distance | Temp.DPE | 32°C__14DPE | 32°C__0DPE | −0.049 | −0.152 | 0.054 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__21DPE | 32°C__0DPE | −0.090 | −0.193 | 0.013 | 0.155 | ns |
| Distance | Temp.DPE | 32°C__28DPE | 32°C__0DPE | −0.081 | −0.188 | 0.026 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__42DPE | 32°C__0DPE | −0.093 | −0.196 | 0.010 | 0.130 | ns |
| Distance | Temp.DPE | 35°C__0DPE | 32°C__0DPE | −0.038 | −0.122 | 0.046 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__14DPE | 32°C__0DPE | −0.082 | −0.189 | 0.025 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__21DPE | 32°C__0DPE | −0.087 | −0.194 | 0.020 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__28DPE | 32°C__0DPE | −0.132 | −0.238 | −0.025 | 0.003 | ** |
| Distance | Temp.DPE | 35°C__42DPE | 32°C__0DPE | 0.024 | −0.087 | 0.135 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__21DPE | 32°C__14DPE | −0.041 | −0.160 | 0.078 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__28DPE | 32°C__14DPE | −0.031 | −0.154 | 0.091 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__42DPE | 32°C__14DPE | −0.043 | −0.162 | 0.076 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__0DPE | 32°C__14DPE | 0.011 | −0.092 | 0.114 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__14DPE | 32°C__14DPE | −0.032 | −0.155 | 0.090 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__21DPE | 32°C__14DPE | −0.037 | −0.160 | 0.085 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__28DPE | 32°C__14DPE | −0.082 | −0.204 | 0.040 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__42DPE | 32°C__14DPE | 0.074 | −0.053 | 0.200 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__28DPE | 32°C__21DPE | 0.010 | −0.113 | 0.132 | ≥0.25 | ns |
| Distance | Temp.DPE | 32°C__42DPE | 32°C__21DPE | −0.002 | −0.121 | 0.117 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__0DPE | 32°C__21DPE | 0.052 | −0.051 | 0.155 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__14DPE | 32°C__21DPE | 0.009 | −0.114 | 0.131 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__21DPE | 32°C__21DPE | 0.004 | −0.119 | 0.126 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__28DPE | 32°C__21DPE | −0.041 | −0.163 | 0.081 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__42DPE | 32°C__21DPE | 0.115 | −0.011 | 0.241 | 0.119 | ns |
| Distance | Temp.DPE | 32°C__42DPE | 32°C__28DPE | −0.012 | −0.134 | 0.110 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__0DPE | 32°C__28DPE | 0.043 | −0.064 | 0.149 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__14DPE | 32°C__28DPE | −0.001 | −0.126 | 0.124 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__21DPE | 32°C__28DPE | −0.006 | −0.132 | 0.119 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__28DPE | 32°C__28DPE | −0.051 | −0.176 | 0.075 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__42DPE | 32°C__28DPE | 0.105 | −0.024 | 0.234 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__0DPE | 32°C__42DPE | 0.054 | −0.049 | 0.157 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__14DPE | 32°C__42DPE | 0.011 | −0.111 | 0.133 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__21DPE | 32°C__42DPE | 0.006 | −0.117 | 0.128 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__28DPE | 32°C__42DPE | −0.039 | −0.161 | 0.083 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__42DPE | 32°C__42DPE | 0.117 | −0.009 | 0.243 | 0.102 | ns |
| Distance | Temp.DPE | 35°C__14DPE | 35°C__0DPE | −0.044 | −0.150 | 0.063 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__21DPE | 35°C__0DPE | −0.049 | −0.155 | 0.058 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__28DPE | 35°C__0DPE | −0.093 | −0.200 | 0.013 | 0.160 | ns |
| Distance | Temp.DPE | 35°C__42DPE | 35°C__0DPE | 0.062 | −0.049 | 0.174 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__21DPE | 35°C__14DPE | −0.005 | −0.130 | 0.120 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__28DPE | 35°C__14DPE | −0.050 | −0.175 | 0.076 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__42DPE | 35°C__14DPE | 0.106 | −0.023 | 0.235 | 0.244 | ns |
| Distance | Temp.DPE | 35°C__28DPE | 35°C__21DPE | −0.045 | −0.170 | 0.081 | ≥0.25 | ns |
| Distance | Temp.DPE | 35°C__42DPE | 35°C__21DPE | 0.111 | −0.018 | 0.240 | 0.180 | ns |
| Distance | Temp.DPE | 35°C__42DPE | 35°C__28DPE | 0.156 | 0.027 | 0.285 | 0.005 | ** |
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Fig. 3 Infection outcomes in zebrafish exposed to Pseudocapillaria tomentosa. (A) Histological sections stained with ### stain in zebrafish exposed to P. tomentosa examined at ### days post exposure. Nematodes in intestinal lumen. Arrow = ###. (B) Infection outcome analysis of fish exposed to P. tomentosa (n = 89) by temperature. Fish reared at 28°C and 32°C water temperatures had significantly different infection burden to fish reared at 35°C water temperature. Only one fish reared at 35°C was identified as being positively infected by wet mount. Only statistically significant relationships are shown. A “*” indicates statistical significance below the “0.05” level.